PROTOCOLS AND NORMALS FOR BOVINE:



ANOVULATION

SAMPLE TIME >>>>>>>>>>>>>>>>>>>>HORMONES TP ASSAY


Anytime>>>>>>>>>>>>>>>>>>>>>>>>>Progesterone, Total T4




ANOVULATION (If iatrogenic Cushing’s is suspected)

SAMPLE TIME >>>>>>>>>>>>>>>>> >>HORMONES TO ASSAY

Sample #1 (Anytime) >> >>>>>>>>>>> >>>>>>>>>>> >Cortisol

>>>>>>>>>>>>>>Administer 80 IU ACTH IM

Sample #2 (90 minutes post ACTH) >>>>>>>>>>>>> >Cortisol

ABORTION

SAMPLE TIME>>>> >>>>>>>>>>>>>>>>HORMONES TO ASSAY


Anytime>>>>>>>>>>>>>>>>>>> >>>>>>Progesterone, Total T4




CYSTIC FOLLICLES

SAMPLE TIME >>>>>>>>>>>>>>>>>>>>HORMONES TO ASSAY


Anytime>>>>>>>>>>>>>>>>>>>Progesterone, Total Estrogens




PITUITARY FUNCTION (GnRH Stimulation Test)

SAMPLE TIME >>>>>>>>>>>>>>>>>>>HORMONES TO ASSAY

Sample #1>>>>>>>>>>>>>>>>>>>>>>> >>>>>>>>>>>LH & FSH

>>>>>>>>>>>>>>Administer 500 mg GnRH

Sample #2 (90 minutes post GnRH) >>>>>>>>>>>>>LH & FSH



NORMAL RANGE FOR BOVINE:

Total T4	30 – 50 ng/mL
Insulin	7 – 20 µI.U./mL
Progesterone:
>> ANESTRUS	< 1.0 ng/mL
>> ESTRUS	< 1.0 ng/mL
>> DIESTRUS	> 3.0 ng/mL
>> PREGNANT	> 3.0 ng/mL